Garlic extracts were obtained and dilution susceptibility assay was used to determine. Methodologies for estimating the antimicrobial activity of go were. Pdf on apr 5, 2014, atheer khashan published antibacterial activity of garlic extract allium sativum against staphylococcus aureus in vitro. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the.
Studies on antimicrobial activity of spices and effect of. The results showed that tr garlic presented more antimicrobial activity than cn garlic. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial. Garlic allium sativum has traditional dietetic and medicinal uses as an antiinfective agent 1. A classroom demonstration of garlic extract and conventional. Ic 50 values observed for the essential oil and ethanol extract were 300. Anti bacterial activity of garlic extract against human pathogenic bacteria.
Antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. Broadspectrum antimicrobial activity, chemical composition. The traditional use of ethiopian garlic for infectious diseases and for controlling fever appears. The extract showed concentration dependent antibacterial activity against staphylococcus aureus.
Jorgensen, map faller, rh yolken manual of clinical microbiology 8th. Fresh garlic extract enhances the antimicrobial activities. Antimicrobial activity of essential oil extracts of various. Antibacterial activity of garlic, tulsi, bitter guard and. It had the highest level of inhibition on the fungus candida albicans garlic, which showed weak antimicrobial. In vitro antibacterial activity and stability of garlic. Garlic allium sativum has attracted considerable attention because of its storage characteristics and durability during shipping. The use of higher plants and preparations from them to treat infections is an age old practice.
Garlic is used in traditional medicine for infectious disease and some other cases. Pdf antimicrobial activity of the extract of garlic and. The results showed that ethanol extract was active against. Giving the concentration of antimicrobial extracts to be 500gml. Garlic has been a favorite additive in food for many years in various cultures. The antibacterial effect of ginger and garlic extracts on. A survey administered after the exercise showed that students gained a better awareness of garlic extracts potency as an antimicrobial agent. Garlic garlic methanolicgm extract garlic ethanolic ge extract tulsi tulsi methanolictm extract. A survey administered after the exercise showed that students gained a better awareness of garlic.
Antibacterial activity of garlic extract on streptomycin. They all have different modes of action by which they act to suppress the infection. To determine the antimicrobial potential of guava psidium guajava leaf extracts against two gramnegative bacteria escherichia coli and salmonella enteritidis and two grampositive bacteria. Mic is generally regarded as the most basic laboratory measurement of the activity of an antimicrobial agent against. Currently, interest has been focused on exploring antimicrobial properties of plants and herbs. Antimicrobial activity of garlic extract against shigella abstract some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or. We set out to determine if pure allicin and aqueous garlic extracts.
Investigation of antioxidant and antimicrobial properties of. Pdf garlic allium sativum l is a species of monocot, bulbforming perennial scented herb belonging to family amaryllidacea. Garlic allium sativum has been used for a long time as a spice or traditional medicine. Department of plant biology, university of ilorin, pmb 1515, ilorin. Antibacterial effect of garlic allium sativum journal of infectious. The methanolic extract of leaf of the plant azadiracta indica, acacia nilotica and witania. Antimicrobial activity of the thiosulfinates isolated from oilmacerated garlic extract created date. Garlic extract exhibited excellent antibacterial activity against all four test organisms while ginger extract showed antibacterial activity. Introduction antimicrobial resistance consequently, antimicrobialresistant. The present study tested the aqueous extract of garlic in vitro for its antibacterial activity. The antioxidant capacity of the spice extracts were found in descending order.
Acetone garlic extracts had antimicrobial activity against 2 of the test microorganisms used s. This study aimed to explore the antimicrobial activity of aqueous garlic extract on. This project work will be limited only to anti microbial activity of garlic and ginger extracts against staphylococcus aureus. Aqueous garlic extract is made up of antioxidant ingredient and has been responsible for boosting the immune system of the body. Chemical composition, antibacterial and antioxidant. Antimicrobial activity of garlic extract against shigella. Antimicrobial activity list of high impact articles ppts. Antimicrobial activity of ginger zingiber officinale. As per ayurvedic classics it possesses wide range of pharmacological action including antimicrobial and anticariogenic. Antimicrobial resistance has been a global concern. Test methodologies were identified as capable of producing underestimates of go activity. The leaf extracts activity being more on bacillus subtilis than aspergillus niger. Antimicrobial activity of garlic extract against shigella abstract some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in gastrointestinal infections.
Various antimicrobial agents are used for this purpose. Some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in gastrointestinal infections. Garlic from nganoini farm in laikipia county, kenya. Garlic has been known to have antibacterial, antifungal, and antiviral activity bakri et al. Pdf antibacterial activity of garlic extract allium. The study aims at determining the antibacterial activity of of. Mic is generally regarded as the most basic laboratory measurement of the activity of an antimicrobial agent against an organism. Clinically, the minimum inhibitory concentrations are used not only to determine the amount of antibiotic that the patient will receive. Antioxidant properties of raw garlic allium sativum extract. Garlic allium sativum has traditional dietetic and medicinal. Garlic peel ethanol extract demonstrated both antioxidant and antibacterial activities in cooked beef. Antibacterial effect of garlic allium sativum against. Antioxidant properties, antimicrobial activity, natural additive, garlic peel. The controls did not show any antimicrobial activity.
It had the highest level of inhibition on the fungus candida albicans garlic, which showed weak antimicrobial effect on the 62 mm, while the lowest level of antimicrobial activity recorded in bacillus subtilis mm table 1. Antimicrobial activity of the thiosulfinates isolated from oilmacerated garlic extract. The purpose of this study is to determine quantitative antimicrobial activity of garlic against shigella by detecting the mic. Students also became enthusiastic about searching for additional sources of antimicrobial. The antimicrobial activities of garlic and other plant alliums are primarily based on allicin, a thiosulphinate present in crushed garlic bulbs. Antibacterial activity of garlic extract on streptomycinresistant.
Many studies have reported significant antiviral activity of some natural extracts, such as the antiviral activity of garlic extract and the fructan from welsh onion against influenza virus type a. Studies have revealed that garlic has activity against bacteria and fungi. The antimicrobial effects of aqueous garlic extracts are well established but those of garlic oil go are little known. Interestingly, comparison of the antimicrobial activity of four independent age preparations showed little batchtobatch variation data not shown. All different samples were prepared and the list is shown in table 1. In addition, larger clear zones were observed at higher concentrations against both microorganisms. The antimicrobial activity from mikania triangularis, known as thin leaf guaco, was tested against five genera of bacteria and three genera of. It was found that most plant extracts studied had antibacterial and antifungal activities. Antimicrobial activity of essential oil extracts of. Grampositive staphylococcus aureus atcc baa 1026 and gramnegative escherichia. Evaluation of antimicrobial activity of different solvent. The methanolic extract of leaf of the plant azadiracta indica, acacia nilotica and witania somnifera showed significant antibacterial activity against bacillus subtilis, escherchia coli, stphaylocuccus aureus and pseudomonas.
Garlic extract has antimicrobial activity against oral bacterial species, particularly gramnegative species. Antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus. Antimicrobial activities of leaf extracts of guava. As observed in the bacterial experiments, the water extracts had the highest activity against plant pathogenic fungi f. Antimicrobial properties of garlic oil against human. On the other hand in our study we have extracted the ginger in soybean which may protect the antimicrobial activity of ginger at high temperature and as a result good antimicrobial activity was found. This study focuses the significant antibacterial activity of garlic allium sativum linn. Different medicinal plants with methanolic and ethanolic extracts. Studies on the antimicrobial properties and phytochemical.
It is a large annual plant of the liliaceae family. Antimicrobial activity list of high impact articles. A comparison of the antimicrobial activity of garlic. In vitro confirmation of the antimicrobial action of fresh and freezedried garlic extracts against human pathogenic bacteria 2, fungi 3, and viruses 4 supports these applications. The inhibitory effect of ginger extract was much higher at all concentrations as compared to garlic extract on the organism. Results of studies on complementary and alternative medicine practices suggest that introduction of plant extracts, such as garlic extract, in antimicrobial. In vitro confirmation of the antimicrobial action of fresh and freezedried garlic extracts against human. The ethyl acetate leaf extract of garlic showed pronounced inhibition than chloroform, petroleum ether and hexane. Garlic is a hardy perennial member of onion family. Antibacterial activity of garlic extract on cariogenic. Antioxidant properties of raw garlic allium sativum extract abstract. Cumin garlic cinnamonturmericginger by dpph method, garlic cuminturmericgingercinnamon by frap method and turmericcinnamon garlic cuminginger by tpc method. However aqueous extract of chinese garlic exhibited better antimicrobial activity than that of pakistani garlic against v. The antibacterial effect of garlic allium safivum and other.
Comparison of antimicrobial activity of allium sativum. Antibacterial activity of garlic extract against human pathogenic. Studies on the antimicrobial properties and phytochemical screening of garlic allium sativum extracts olusanmi, m. Antimicrobial activity of the extract of garlic and onions. In vitro antimicrobial activity of garlic allium sativum. H7 in laboratory buffer and model food system were investigated.
Moore and atkins reported that the inhibitory property of garlic extract was unaffected by storage. Investigation of antioxidant and antimicrobial properties. Antimicrobial activity was tested by using 3 different processes chopping, freezing, and slicing by the disk diffusion method. Ginger, garlic, extract, pathogenic bacteria, otitis media. Antimicrobial properties of garlic oil against human enteric. Garlic extract exhibits broad spectrum antimicrobial activity against many genera of bacteria and fungi at room temperature 14. Garlic extract exhibited excellent antibacterial activity against all four test. Introduction ginger ginger rhizome is the root of the zingiber officinale. Garlic contains allicin compound which has the role as antiinfective agents. In vitro antibacterial activity of aquatic garlic extract. H2 garlic and ginger extracts mixed show enhanced anti microbial activity against staphylococcus aureus. We set out to determine if pure allicin and aqueous garlic extracts age exhibit antimicrobial properties against the burkholderia cepacia complex bcc, the major bacterial phytopathogen for alliums and an intrinsically multiresistant and life.
Comparison of antimicrobial activity of allium sativum cloves. The data presented in table 1 shows that garlic aqueous extract exhibited highest antibacterial activity against all tested bacteria except e. Antimicrobial may be antibacterial, antifungal or antiviral. Inhibitory effect of allium sativum and zingiber officinale. Pdf antimicrobial activity of garlic extract against. Age displayed antimicrobial activity against all of the 38 bcc isolates table 1 with mics ranging from 0. Pdf antimicrobial activity of garlic extract on organisms.
The present study deals with the study of antibacterial activity of various spices. The antimicrobial effects of garlic, ginger, carrot and turmeric pastes against escherichia coli o157. This project work will be limited only to anti microbial. In india, garlic has been used to prevent wound infection and food spoilage. Pdf antiviral activity of garlic extract on influenza virus. Louis pasteur was the first described the antibacterial effect of onion and garlic juices against both grampositive and gramnegative bacteria.
The purpose of this study is to determine quantitative antimicrobial activity of garlic against. Antibacterial activity of garlic allium sativum extract. H1 ginger and garlic extracts do not show anti microbial activity on staphylococcus aureus. Antioxidant properties, antimicrobial activity, natural additive, garlic peel extract 1. Antioxidant activity and antimicrobial property of some. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science in the subject life sciences at the university of south africa supervisor. Pdf antibacterial activity of garlic extract allium sativum against. Methodologies for estimating the antimicrobial activity of go were assessed and go, go sulfide constituents, and garlic powder gp were compared in tests against human enteric bacteria. Introduction garlic is widely used around the world for its pungent flavor as a seasoning or condiment.
The antibacterial activity of the crude extract of garlic was. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by two variants of the agar diffusion. Moore and atkins reported that the inhibitory property of garlic extract was unaffected by storage temperatures,but the fungicidal properties of the same garlic preparation decreased at different temperatures. Apr 27, 2012 the controls did not show any antimicrobial activity. The results showed that tr garlic presented more antimicrobial activity than. Antimicrobial properties of the extracts of locally sold. The essential oil and ethanol extract from raw garlic were tested for antimicrobial activity against seven microorganisms. Garlic methanol extract was least effective against all tested bacteria. Garlic was rated as being equal to, or less in inhibition than the antibiotics. The toxicity of extracts from arthemus sativa, which is known to have antimicrobial activity, was also studied 10. A study of antimicrobial activity of few medicinal herbs. According to the results, garlic extracts exhibited antimicrobial activity against all of seven bacteria, and these results suggest that this garlic extract can be used as an antimicrobial.
On the other hand in our study we have extracted the ginger in soybean which may protect the antimicrobial activity of ginger at high temperature and as a result good antimicrobial activity was. The results indicated that garlic absolute ethanol extract had the highest. Antimicrobial activity of the thiosulfinates isolated from. Some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in. Antimicrobial activity refers to the process of killing or inhibiting the disease causing microbes. To evaluate the antimicrobial activity of fresh juice of. Mechanism of activity of cn garlic could be proposed to be different from that of tr garlic.
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